| Claude Bernard - Physiologist and Founder | Owner of the premises, central figure of the museum. |
| Fanny Martin - Wife of Claude Bernard | SPA activist, opposed to his experiments. |
| Marie-Sarah Raffalovich - Friend and confidant | Polyglot, helped disseminate its work. |
| François Magendie - Scientific Mentor | His professor at the Collège de France. |
| Paul Bert - Disciple and successor | Heir of his laboratory and methods. |