| Jean-Louis Rast-Maupas - Founder | Created the first private condition in 1779. |
| Joseph-Jean-Pascal Gay - Architect | Designed the building between 1804 and 1814. |
| Léon Talabot - Engineer | Perfected the desiccation method in 1842. |
| Jules Dusuzeau - Director of the Laboratory | Directed research on silk (1885–97). |
| Daniel Levrat - Director of the Laboratory | Successor of Dusuzeau, stationed until 1940. |
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