| Gustave Adolphe Thuret - Botanist and founder | Creator of the garden in 1858. |
| Édouard Bornet - Scientific Collaborator | Continues work after 1875. |
| Louise Fould - Benefactor | Give the Villa to the State. |
| Georges Poirault - Director and restaurant | Relaunched the garden in 1899. |
| Charles Naudin - First Official Director | Named in 1878. |