| Guy de La Brosse - King's doctor and founder | Created the Royal Garden of Medicinal Plants in 1626. |
| Georges-Louis Leclerc de Buffon - Naturalist and intendant | Directed the King's Garden from 1739 to 1788. |
| Georges Cuvier - Naturalist and anatomist | Pioneer of comparative anatomy and paleontology. |
| Étienne Geoffroy Saint-Hilaire - Zoologist and transformist | Founded the menagerie and defended evolutionary theories. |
| Jean-Baptiste de Lamarck - Naturalist and biologist | Theoretician of species evolution. |
| Joseph Lakanal - Politician and founder | Proposed the creation of the Museum in 1793. |